| GMP-grade pneumococcal whole-cell vaccine injected subcutaneously protects mice from nasopharyngeal colonization and fatal aspiration-sepsis YJ Lu, L Leite, VM Gonçalves, W de Oliveira Dias, C Liberman, F Fratelli, ... Vaccine 28 (47), 7468-7475, 2010 | 110 | 2010 |
| Pulmonary dry powder vaccine of pneumococcal antigen loaded nanoparticles NK Kunda, IM Alfagih, EN Miyaji, DB Figueiredo, VM Gonçalves, ... International journal of pharmaceutics 495 (2), 903-912, 2015 | 75 | 2015 |
| Mucosal immunization with PspA (Pneumococcal surface protein A)-adsorbed nanoparticles targeting the lungs for protection against pneumococcal infection TC Rodrigues, MLS Oliveira, A Soares-Schanoski, SL Chavez-Rico, ... PloS one 13 (1), e0191692, 2018 | 62 | 2018 |
| Cost analysis based on bioreactor cultivation conditions: Production of a soluble recombinant protein using Escherichia coli BL21 (DE3) VM Cardoso, G Campani, MP Santos, GG Silva, MC Pires, VM Gonçalves, ... Biotechnology reports 26, e00441, 2020 | 60 | 2020 |
| Purification of capsular polysaccharide from Streptococcus pneumoniae serotype 23F by a procedure suitable for scale‐up VMM Gonçalves, M Takagi, RB Lima, H Massaldi, RC Giordano, ... Biotechnology and applied biochemistry 37 (3), 283-287, 2003 | 58 | 2003 |
| Optimization of medium and cultivation conditions for capsular polysaccharide production by Streptococcus pneumoniae serotype 23F VM Gonçalves, TC Zangirolami, RLC Giordano, I Raw, MM Tanizaki, ... Applied microbiology and biotechnology 59, 713-717, 2002 | 50 | 2002 |
| Development of production and purification processes of recombinant fragment of pneumococcal surface protein A in Escherichia coli using different carbon … RJ Carvalho, J Cabrera-Crespo, MM Tanizaki, VM Gonçalves Applied microbiology and biotechnology 94, 683-694, 2012 | 45 | 2012 |
| Development of a whole cell pneumococcal vaccine: BPL inactivation, cGMP production, and stability VM Gonçalves, WO Dias, IB Campos, C Liberman, ME Sbrogio-Almeida, ... Vaccine 32 (9), 1113-1120, 2014 | 41 | 2014 |
| Conjugation of polysaccharide 6B from Streptococcus pneumoniae with pneumococcal surface protein A: PspA conformation and its effect on the immune response CT Perciani, GC Barazzone, C Goulart, E Carvalho, J Cabrera-Crespo, ... Clinical and Vaccine Immunology 20 (6), 858-866, 2013 | 35 | 2013 |
| Optimization and scale-up production of Zika virus ΔNS1 in Escherichia coli: application of Response Surface Methodology AI Kanno, LCC Leite, LR Pereira, MJR de Jesus, R Andreata-Santos, ... Amb Express 10, 1-13, 2020 | 34 | 2020 |
| On-line monitoring of biomass concentration based on a capacitance sensor: assessing the methodology for different bacteria and yeast high cell density fed-batch cultures ACL Horta, AJ Silva, CR Sargo, ID Cavalcanti-Montaño, ... Brazilian Journal of Chemical Engineering 32 (4), 821-829, 2015 | 32 | 2015 |
| Intensification of high cell-density cultivations of rE. coli for production of S. pneumoniae antigenic surface protein, PspA3, using model-based adaptive control ACL Horta, CR Sargo, AJ da Silva, M de Carvalho Gonzaga, ... Bioprocess and biosystems engineering 35, 1269-1280, 2012 | 32 | 2012 |
| Protection induced by pneumococcal surface protein A (PspA) is enhanced by conjugation to a Streptococcus pneumoniae capsular polysaccharide FCL Csordas, CT Perciani, M Darrieux, VM Goncalves, J Cabrera-Crespo, ... Vaccine 26 (23), 2925-2929, 2008 | 32 | 2008 |
| Albumin purification from human placenta J Cabrera‐Crespo, VM Gonçalves, EAL Martins, S Grellet, APY Lopes, ... Biotechnology and applied biochemistry 31 (2), 101-106, 2000 | 31 | 2000 |
| Anti-Flavivirus Vaccines: Review of the Present Situation and Perspectives of Subunit Vaccines Produced in Escherichia coli SC Araujo, LR Pereira, RPS Alves, R Andreata-Santos, AI Kanno, ... Vaccines 8 (3), 492, 2020 | 28 | 2020 |
| A supervision and control tool based on artificial intelligence for high cell density cultivations ACL Horta, AJ Silva, CR Sargo, AM Velez, MC Gonzaga, RC Giordano, ... Brazilian Journal of Chemical Engineering 31, 457-468, 2014 | 25 | 2014 |
| Crianças pequenas e seus conhecimentos sobre microrganismos [Young children and their knowledge of microorganisms] A Bizerra, C Dominguez, G Inglez, V Goncalves, N Leporo, M Franco Encontro Nacional em Pesquisa em Educação em Ciências 7, 2009 | 25 | 2009 |
| Simple and efficient method of bacterial polysaccharides purification for vaccines production using hydrolytic enzymes and tangential flow ultrafiltration VM Gonçalves, M Takagi, TS Carmo, RB Lima, SMF Albani, JV Pinto, ... Communicating current research and educational topics and trends in applied …, 2007 | 23 | 2007 |
| IL-17A and complement contribute to killing of pneumococci following immunization with a pneumococcal whole cell vaccine IB Campos, M Herd, KL Moffitt, YJ Lu, M Darrieux, R Malley, LCC Leite, ... Vaccine 35 (9), 1306-1315, 2017 | 22 | 2017 |
| Production and purification of an untagged recombinant pneumococcal surface protein A (PspA4Pro) with high-purity and low endotoxin content DB Figueiredo, E Carvalho, MP Santos, S Kraschowetz, RT Zanardo, ... Applied microbiology and biotechnology 101, 2305-2317, 2017 | 22 | 2017 |
