| Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization JM Bolivar, JM Woodley, R Fernandez-Lafuente Chemical Society Reviews 51 (15), 6251-6290, 2022 | 225 | 2022 |
| Advanced characterization of immobilized enzymes as heterogeneous biocatalysts JM Bolivar, I Eisl, B Nidetzky Catalysis Today 259, 66-80, 2016 | 191 | 2016 |
| Biotransformations in microstructured reactors: more than flowing with the stream? JM Bolivar, J Wiesbauer, B Nidetzky Trends in biotechnology 29 (7), 333-342, 2011 | 173 | 2011 |
| Coating of soluble and immobilized enzymes with ionic polymers: full stabilization of the quaternary structure of multimeric enzymes JM Bolivar, J Rocha-Martin, C Mateo, F Cava, J Berenguer, ... Biomacromolecules 10 (4), 742-747, 2009 | 129 | 2009 |
| Improvement of enzyme properties with a two-step immobilizaton process on novel heterofunctional supports C Mateo, JM Bolivar, CA Godoy, J Rocha-Martin, BC Pessela, JA Curiel, ... Biomacromolecules 11 (11), 3112-3117, 2010 | 119 | 2010 |
| Improvement of the stability of alcohol dehydrogenase by covalent immobilization on glyoxyl-agarose JM Bolivar, L Wilson, SA Ferrarotti, JM Guisan, R Fernandez-Lafuente, ... Journal of biotechnology 125 (1), 85-94, 2006 | 113 | 2006 |
| Stabilization of a formate dehydrogenase by covalent immobilization on highly activated glyoxyl-agarose supports JM Bolivar, L Wilson, SA Ferrarotti, R Fernandez-Lafuente, JM Guisan, ... Biomacromolecules 7 (3), 669-673, 2006 | 108 | 2006 |
| Characterization and evaluation of immobilized enzymes for applications in flow reactors JM Bolivar, F López-Gallego Current Opinion in Green and Sustainable Chemistry 25, 100349, 2020 | 94 | 2020 |
| Positively charged mini-protein zbasic2 as a highly efficient silica binding module: Opportunities for enzyme immobilization on unmodified silica supports JM Bolivar, B Nidetzky Langmuir 28 (26), 10040-10049, 2012 | 90 | 2012 |
| Evaluation of different immobilization strategies to prepare an industrial biocatalyst of formate dehydrogenase from Candida boidinii JM Bolivar, L Wilson, SA Ferrarotti, R Fernandez-Lafuente, JM Guisan, ... Enzyme and microbial technology 40 (4), 540-546, 2007 | 81 | 2007 |
| Shine a light on immobilized enzymes: real-time sensing in solid supported biocatalysts JM Bolivar, T Consolati, T Mayr, B Nidetzky Trends in biotechnology 31 (3), 194-203, 2013 | 75 | 2013 |
| Purification and stabilization of a glutamate dehygrogenase from Thermus thermophilus via oriented multisubunit plus multipoint covalent immobilization JM Bolivar, J Rocha-Martin, C Mateo, F Cava, J Berenguer, D Vega, ... Journal of Molecular Catalysis B: Enzymatic 58 (1-4), 158-163, 2009 | 72 | 2009 |
| The science of enzyme immobilization JM Guisan, F López-Gallego, JM Bolivar, J Rocha-Martín, ... Immobilization of Enzymes and Cells: Methods and Protocols, 1-26, 2020 | 71 | 2020 |
| Oriented and selective enzyme immobilization on functionalized silica carrier using the cationic binding module Zbasic2: Design of a heterogeneous D‐amino acid oxidase catalyst … JM Bolivar, B Nidetzky Biotechnology and bioengineering 109 (6), 1490-1498, 2012 | 67 | 2012 |
| Modulation of the distribution of small proteins within porous matrixes by smart-control of the immobilization rate JM Bolivar, A Hidalgo, L Sánchez-Ruiloba, J Berenguer, JM Guisán, ... Journal of biotechnology 155 (4), 412-420, 2011 | 66 | 2011 |
| Heterofunctional supports for the one-step purification, immobilization and stabilization of large multimeric enzymes: Amino-glyoxyl versus amino-epoxy supports JM Bolivar, C Mateo, V Grazu, AV Carrascosa, BC Pessela, JM Guisan Process Biochemistry 45 (10), 1692-1698, 2010 | 65 | 2010 |
| Multiphase biotransformations in microstructured reactors: opportunities for biocatalytic process intensification and smart flow processing JM Bolivar, B Nidetzky Green Processing and Synthesis 2 (6), 541-559, 2013 | 61 | 2013 |
| The presence of thiolated compounds allows the immobilization of enzymes on glyoxyl agarose at mild pH values: New strategies of stabilization by multipoint covalent attachment JM Bolivar, F López-Gallego, C Godoy, DS Rodrigues, RC Rodrigues, ... Enzyme and Microbial Technology 45 (6-7), 477-483, 2009 | 61 | 2009 |
| Immobilization of proteins on highly activated glyoxyl supports: Dramatic increase of the enzyme stability via multipoint immobilization on pre-existing carriers G Fernandez-Lorente, F Lopez-Gallego, J M Bolivar, J Rocha-Martin, ... Current Organic Chemistry 19 (17), 1719-1731, 2015 | 60 | 2015 |
| New biotechnological perspectives of a NADH oxidase variant from Thermus thermophilus HB27 as NAD+-recycling enzyme J Rocha-Martín, D Vega, JM Bolivar, CA Godoy, A Hidalgo, J Berenguer, ... BMC biotechnology 11, 1-11, 2011 | 60 | 2011 |
